Repair of UV-damaged DNA by DNA photolyase and cryptochrome (single-strand DNA photolyase).
Antibiotic activation and resistance in KatG, the catalase-peroxidase of Mycobacterium tuberculosis.
The exposure of cells to solar light, especially UV-A through UV-C light induces damage in DNA. This damage is mainly the dimerization of adjacent pyrimidine bases (cytidine and thymidine) in DNA. In this project, we are interested in characterizing these dimers by applying various spectroscopic techniques such as NMR, UV-vis absorption, Raman, and FT-IR spectroscopy. The spectroscopic methods are complemented with computational chemistry to obtain a better understanding of the spectroscopic data. Since the irradiation of DNA and DNA bases in solution with UV-light results in the formation of several photoproducts, we also use HPLC to purify the photodimers of interest. Besides studying small strands of DNA, we also investigate the nucleobases and nucleotides in solution.