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Nikon Confocal Microscope Training Session

August 10, 2016, 1:00 pm - 2:00 pm
Location Center for Environmental and Life Sciences - 110
Posted InCollege of Science and Mathematics
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Will be presented by a Nikon application specialist

Nikon C2 Plus Confocal Laser Scanning Microscope (CLSM): The Nikon C2 Plus is a point scanning confocal microscope with 4 channels: 405, 488, 561 and 640nm excitation. It is equipped with 4 solid state laser lines (405, 488, 561 and 640 nm) and is able to take images of up to 2048 x 2048 µm pixels resolution. It provides an “X-cite” 120 LED system is for fluorescence and filters for widefield using DAPI, GFP, and Tex Red filters. The objectives for the Ti system are 10x, 20x, 40x, and 60x oil, all with DIC capabilities.  Detectors include 3 standard Photo-multiplier Tubes (PMTs), plus a transmitted detector.  The scan head is attached to a fully automated Nikon Ti microscope and entirely controlled by the NIS-Elements C software. Extended life experiments are facilitated by a Nikon Perfect Focus System that continuously determines the distance to the coverslip. 

Objectives

  • Nikon Plan Apo 10x NA 0.45
  • Nikon Plan Apo 20x NA 0.75
  • Nikon Plan Fluor 40x NA 0.75  
  • Nikon Apo λ (Lambda) 60x NA 1.40 oil immersion (working distance 0.13mm)