Leica microscope in a dark room

Policies and Rates

Personal use of microscopes is open only to those taking a training course or academic credit course in electron microscopy. Non-qualified users operating equipment will result in loss of usage privileges of the facility.

MMRL Schedule

The regular hours of the MMRL will be from 8:30 a.m. to 5:00 p.m. Monday through Friday (9:00 a.m. to 4:30 p.m. if the University is on the summer schedule). The facility will be open at other times only to qualified independent night operators or by special arrangement with the MMRL Director/Co-director or instructors.

Instrument scheduling

In order to permit as many researchers and outreach groups as possible to use the instruments and to help researchers plan their time for using the MMRL, a web-based instrument calendar and sign-up system has been set up. Users have to schedule in advance up to four-hour blocks of time in a day. Scheduling will be done on a weekly basis.

If scheduled maintenance/repair or if time for training is needed on an instrument, notice will be posted.

Scheduling training and assistance

If a training or assistance is needed, the researcher should contact the MMRL Director, Laying Wu, a week or two (the earlier the better) prior to needing the microscopy work.

No-shows

Anyone scheduled to use the MMRL facilities is expected to do so at the assigned time. If one cannot come to the MMRL or the sample is not ready, he/she must contact the EM staff in advance to cancel the reservation. Repeatedly missing your appointment time may lead to privileges for use of the MMRL facilities to be restricted.

Log Books

Log books are provided for each piece of equipment in the MMRL. Each time a piece of equipment is utilized, an entry must be placed in the log sheet. Entries will vary, but will include the name of the user, user’s advisor, date, and time (starting and ending) of use and comments. It is essential that these logs be maintained to facilitate instrument maintenance.

Rates

Hourly charges for internal, external academic and external industrial users will be calculated for the equipment’s maintenance and supply costs. Cost basis includes:

  1. Direct usage and specimen preparation costs.
  2. Supplies required to prepare sample(s).
  3. Supplies to maintain the instruments.
  4. Service contracts.
  5. Administrative costs.

Failure to make payments may result in loss of privileges in the MMRL until the charge is paid.

Internal Educational

Internal Educational
Equipment Internal Academic
Scanning Electron Microscopy (Cost Per Run)
Hitachi S-3400N VP SEM 20
Hitachi S-2460 SEM 15
Transmission Electron Microscopy (Cost Per Run)
Hitachi H-7500 TEM 20
Scanning Probe Microscope (Cost Per Run)
Angstrom AA5000 SPM 20
Confocal Laser Scanning Microscope (Cost Per Run)
Nikon C2 CLSM 25
Microtomy (Cost Per Run)
Thick Sectioning 20
Ultrathin Sectioning 20
Auxiliary Facilities (Cost Per Run)
Critical point drying (CO2 costs and cylinder costs) 10
Sputter coating (Metal target, Argon and cylinder costs) (Denton Desk II) 10
Carbon coating/shadow coating (Denton DV 502A high vacuum evaporator) 10
Fast carbon coating (Denton Desk IV) 10
Tissue processor 20
Imaging analysis
Sample preparation costs (Per Sample)
Biological samples for SEM (including conventional fixation and dehydration) 15
Process and embedding in resin (including: fixation, dehydration, infiltration) and: 20
Tissue samples for paraffin embedding 10
Negative staining or double staining 5
Immunolabeling Varies
Histochemical staining (H and E stain) 8
Histochemical staining (special stain) Varies
Support (Cost Per Hour)
Technical Support 20
Rush order technical support 40
Imaging analysis 10
Supplies (Cost Per Grid)
Osmium tetroxide (2mL 4% in water) 6
Formvar coated or carbon coated grids 3/grid
Lacey carbon grids 6/grid

External Educational

Equipment External Educational
Scanning Electron Microscopy (Cost Per Run)
Hitachi S-3400N VP SEM 45
Hitachi S-2460 SEM 35
Transmission Electron Microscopy (Cost Per Run)
Hitachi H-7500 TEM 55
Scanning Probe Microscope (Cost Per Run)
Angstrom AA5000 SPM 40
Confocal Laser Scanning Microscope (Cost Per Run)
Nikon C2 CLSM 55
Microtomy (Cost Per Run)
Thick Sectioning 35
Ultrathin Sectioning 40
Auxiliary Facilities (Cost Per Run)
Critical point drying (CO2 costs and cylinder costs) 15
Sputter coating (Metal target, Argon and cylinder costs) (Denton Desk II) 15
Carbon coating/shadow coating (Denton DV 502A high vacuum evaporator) 15
Fast carbon coating (Denton Desk IV) 20
Tissue processor 60
Imaging analysis
Sample preparation costs (Per Sample)
Biological samples for SEM (including conventional fixation and dehydration) 25
Process and embedding in resin (including: fixation, dehydration, infiltration) and: 50
Tissue samples for paraffin embedding 10
Negative staining or double staining 8
Immunolabeling Varies
Histochemical staining (H and E stain) 20
Histochemical staining (special stain) Varies
Support (Cost Per Hour)
Technical Support 30
Rush order technical support 40
Imaging analysis 15
Supplies (Cost Per Grid)
Osmium tetroxide (2mL 4% in water) 6
Formvar coated or carbon coated grids 3/grid
Lacey carbon grids 6/grid

External Non-Educational

External Non-Educational
Equipment External Non-Educational
Scanning Electron Microscopy (Cost Per Run)
Hitachi S-3400N VP SEM 150
Hitachi S-2460 SEM 150
Transmission Electron Microscopy (Cost Per Run)
Hitachi H-7500 TEM 150
Scanning Probe Microscope (Cost Per Run)
Angstrom AA5000 SPM 150
Confocal Laser Scanning Microscope (Cost Per Run)
Nikon C2 CLSM 120
Microtomy (Cost Per Run)
Thick Sectioning 40
Ultrathin Sectioning 60
Auxiliary Facilities (Cost Per Run)
Critical point drying (CO2 costs and cylinder costs) 20
Sputter coating (Metal target, Argon and cylinder costs) (Denton Desk II) 20
Carbon coating/shadow coating (Denton DV 502A high vacuum evaporator) 30
Fast carbon coating (Denton Desk IV) 80
Tissue processor 30/hour
Imaging analysis 30/hour
Sample preparation costs (Per Sample)
Biological samples for SEM (including conventional fixation and dehydration) 50
Process and embedding in resin (including: fixation, dehydration, infiltration) and: 50
Tissue samples for paraffin embedding 60/hour
Negative staining or double staining 10
Immunolabeling Varies
Histochemical staining (H and E stain) 40
Histochemical staining (special stain) Varies
Support (Cost Per Hour)
Technical Support
Rush order technical support
Imaging analysis
Supplies (Cost Per Grid)
Osmium tetroxide (2mL 4% in water)
Formvar coated or carbon coated grids
Lacey carbon grids