Personal use of microscopes is open only to those taking a training course or academic credit course in electron microscopy. Non-qualified users operating equipment will result in loss of usage privileges of the facility.
MMRL Schedule
The regular hours of the MMRL will be from 8:30 a.m. to 5:00 p.m. Monday through Friday (9:00 a.m. to 4:30 p.m. if the University is on the summer schedule). The facility will be open at other times only to qualified independent night operators or by special arrangement with the MMRL Director/Co-director or instructors.
Instrument scheduling
In order to permit as many researchers and outreach groups as possible to use the instruments and to help researchers plan their time for using the MMRL, a web-based instrument calendar and sign-up system has been set up. Users have to schedule in advance up to four-hour blocks of time in a day. Scheduling will be done on a weekly basis.
If scheduled maintenance/repair or if time for training is needed on an instrument, notice will be posted.
Scheduling training and assistance
If a training or assistance is needed, the researcher should contact the MMRL Director, Laying Wu, a week or two (the earlier the better) prior to needing the microscopy work.
No-shows
Anyone scheduled to use the MMRL facilities is expected to do so at the assigned time. If one cannot come to the MMRL or the sample is not ready, he/she must contact the EM staff in advance to cancel the reservation. Repeatedly missing your appointment time may lead to privileges for use of the MMRL facilities to be restricted.
Log Books
Log books are provided for each piece of equipment in the MMRL. Each time a piece of equipment is utilized, an entry must be placed in the log sheet. Entries will vary, but will include the name of the user, user’s advisor, date, and time (starting and ending) of use and comments. It is essential that these logs be maintained to facilitate instrument maintenance.
Rates
Hourly charges for internal, external academic and external industrial users will be calculated for the equipment’s maintenance and supply costs. Cost basis includes:
- Direct usage and specimen preparation costs.
- Supplies required to prepare sample(s).
- Supplies to maintain the instruments.
- Service contracts.
- Administrative costs.
Failure to make payments may result in loss of privileges in the MMRL until the charge is paid.
Internal Educational
Equipment | Internal Academic | ||
---|---|---|---|
Scanning Electron Microscopy (Cost Per Run) | |||
Hitachi S-3400N VP SEM | 20 | ||
Hitachi S-2460 SEM | 15 | ||
Transmission Electron Microscopy (Cost Per Run) | |||
Hitachi H-7500 TEM | 20 | ||
Scanning Probe Microscope (Cost Per Run) | |||
Angstrom AA5000 SPM | 20 | ||
Confocal Laser Scanning Microscope (Cost Per Run) | |||
Nikon C2 CLSM | 25 | ||
Microtomy (Cost Per Run) | |||
Thick Sectioning | 20 | ||
Ultrathin Sectioning | 20 | ||
Auxiliary Facilities (Cost Per Run) | |||
Critical point drying (CO2 costs and cylinder costs) | 10 | ||
Sputter coating (Metal target, Argon and cylinder costs) (Denton Desk II) | 10 | ||
Carbon coating/shadow coating (Denton DV 502A high vacuum evaporator) | 10 | ||
Fast carbon coating (Denton Desk IV) | 10 | ||
Tissue processor | 20 | ||
Imaging analysis | |||
Sample preparation costs (Per Sample) | |||
Biological samples for SEM (including conventional fixation and dehydration) | 15 | ||
Process and embedding in resin (including: fixation, dehydration, infiltration) and: | 20 | ||
Tissue samples for paraffin embedding | 10 | ||
Negative staining or double staining | 5 | ||
Immunolabeling | Varies | ||
Histochemical staining (H and E stain) | 8 | ||
Histochemical staining (special stain) | Varies | ||
Support (Cost Per Hour) | |||
Technical Support | 20 | ||
Rush order technical support | 40 | ||
Imaging analysis | 10 | ||
Supplies (Cost Per Grid) | |||
Osmium tetroxide (2mL 4% in water) | 6 | ||
Formvar coated or carbon coated grids | 3/grid | ||
Lacey carbon grids | 6/grid |
External Educational
Equipment | External Educational | ||
---|---|---|---|
Scanning Electron Microscopy (Cost Per Run) | |||
Hitachi S-3400N VP SEM | 45 | ||
Hitachi S-2460 SEM | 35 | ||
Transmission Electron Microscopy (Cost Per Run) | |||
Hitachi H-7500 TEM | 55 | ||
Scanning Probe Microscope (Cost Per Run) | |||
Angstrom AA5000 SPM | 40 | ||
Confocal Laser Scanning Microscope (Cost Per Run) | |||
Nikon C2 CLSM | 55 | ||
Microtomy (Cost Per Run) | |||
Thick Sectioning | 35 | ||
Ultrathin Sectioning | 40 | ||
Auxiliary Facilities (Cost Per Run) | |||
Critical point drying (CO2 costs and cylinder costs) | 15 | ||
Sputter coating (Metal target, Argon and cylinder costs) (Denton Desk II) | 15 | ||
Carbon coating/shadow coating (Denton DV 502A high vacuum evaporator) | 15 | ||
Fast carbon coating (Denton Desk IV) | 20 | ||
Tissue processor | 60 | ||
Imaging analysis | |||
Sample preparation costs (Per Sample) | |||
Biological samples for SEM (including conventional fixation and dehydration) | 25 | ||
Process and embedding in resin (including: fixation, dehydration, infiltration) and: | 50 | ||
Tissue samples for paraffin embedding | 10 | ||
Negative staining or double staining | 8 | ||
Immunolabeling | Varies | ||
Histochemical staining (H and E stain) | 20 | ||
Histochemical staining (special stain) | Varies | ||
Support (Cost Per Hour) | |||
Technical Support | 30 | ||
Rush order technical support | 40 | ||
Imaging analysis | 15 | ||
Supplies (Cost Per Grid) | |||
Osmium tetroxide (2mL 4% in water) | 6 | ||
Formvar coated or carbon coated grids | 3/grid | ||
Lacey carbon grids | 6/grid |
External Non-Educational
Equipment | External Non-Educational | ||
---|---|---|---|
Scanning Electron Microscopy (Cost Per Run) | |||
Hitachi S-3400N VP SEM | 150 | ||
Hitachi S-2460 SEM | 150 | ||
Transmission Electron Microscopy (Cost Per Run) | |||
Hitachi H-7500 TEM | 150 | ||
Scanning Probe Microscope (Cost Per Run) | |||
Angstrom AA5000 SPM | 150 | ||
Confocal Laser Scanning Microscope (Cost Per Run) | |||
Nikon C2 CLSM | 120 | ||
Microtomy (Cost Per Run) | |||
Thick Sectioning | 40 | ||
Ultrathin Sectioning | 60 | ||
Auxiliary Facilities (Cost Per Run) | |||
Critical point drying (CO2 costs and cylinder costs) | 20 | ||
Sputter coating (Metal target, Argon and cylinder costs) (Denton Desk II) | 20 | ||
Carbon coating/shadow coating (Denton DV 502A high vacuum evaporator) | 30 | ||
Fast carbon coating (Denton Desk IV) | 80 | ||
Tissue processor | 30/hour | ||
Imaging analysis | 30/hour | ||
Sample preparation costs (Per Sample) | |||
Biological samples for SEM (including conventional fixation and dehydration) | 50 | ||
Process and embedding in resin (including: fixation, dehydration, infiltration) and: | 50 | ||
Tissue samples for paraffin embedding | 60/hour | ||
Negative staining or double staining | 10 | ||
Immunolabeling | Varies | ||
Histochemical staining (H and E stain) | 40 | ||
Histochemical staining (special stain) | Varies | ||
Support (Cost Per Hour) | |||
Technical Support | |||
Rush order technical support | |||
Imaging analysis | |||
Supplies (Cost Per Grid) | |||
Osmium tetroxide (2mL 4% in water) | |||
Formvar coated or carbon coated grids | |||
Lacey carbon grids |